Livability of Bovine Spermatozoa at 5 C in Tris-Buffered and Citrate-Buffered Yolk-Glycerol Extenders
نویسندگان
چکیده
منابع مشابه
Influence of Added Vitamin C and Vitamin E on Frozen-Thawed Bovine Sperm Cryopreserved in Citrate and Tris-Based Extenders
The objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin C and E as antioxidants. Pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/mL with citrate –egg yolk (CEY) or Tris – egg yolk (TEY) extenders. The diluted semen was divided to 5 aliquots including control and 4 experimental groups....
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A modified Tris-buffered medium (mTBM) has been widely used as an insemination medium for porcine in vitro fertilization (IVF). We examined whether mTBM could be used for bovine IVF. Bovine cumulus-oocyte complexes (COCs) were cultured in a serum-free medium containing 30 ng/ml EGF for 22 h. After culture, COCs were inseminated with spermatozoa for 12 h in mTBM containing 5 mM caffeine and 10 g...
متن کاملInfluence of glycerol levels, diluent and post-thaw temperature on motility and acrosomal maintenance in bovine semen frozen in plastic straws.
One ejaculate from each Of 13 bulls was used to determine the influence of glycerol level on post-thaw susceptibili ty of spermatozoa in French straws to cold shock. Treatments were arranged factorially and included yolk-Tris and yolk-citrate diluents; 7, 9 and 11% glycerol ;and exposure to 1, 20 or 37 C water baths for 3 rain immediately post-thaw. Percentage of motile spermatozoa and percenta...
متن کاملinfluence of added vitamin c and vitamin e on frozen-thawed bovine sperm cryopreserved in citrate and tris-based extenders
the objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin c and e as antioxidants. pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/ml with citrate –egg yolk (cey) or tris – egg yolk (tey) extenders. the diluted semen was divided to 5 aliquots including control and 4 experimental groups....
متن کاملEffect of Different Levels of Egg Yolk on Cryopreservation of Black Bengal Buck Semen in Tris Egg yolk Citrate Fructose Glycerol Extender
This study was conducted to assess the physical and morphological characteristics of fresh, pre-freeze and post-thaw black Bengal buck (Capra hircus) semen processed in Tris-egg yolk-citrate-fructose-glycerol (TEYCFG) extender containing 4 different concentrations (2.5, 5, 7.5 and 10%) of egg-yolk. Ejaculates were collected once a week for eight weeks from six mature bucks (48 ejaculates) using...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1963
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(63)88964-x